Analisis Efek Terapi Kombinasi Beras Analog dan Glimepirid terhadap Kadar MDA Pankreas pada Tikus Diabetes Melitus Tipe 2
DOI:
https://doi.org/10.20527/jps.v11i2.18891Kata Kunci:
Type 2 Diabetes Mellitus, MDA Pancreas, Glimepiride, Analog Rice, MOCAFAbstrak
Prevalensi dan insidensi diabetes melitus tipe 2 (DMT 2) di dunia terus meningkat. Penyakit ini ditandai dengan hiperglikemia yang memicu kondisi stres oksidatif. Salah satu terapi oral yang sering digunakan adalah glimepirid. Pengendalian hiperglikemia pada penderita DM Tipe 2 membutuhkan perubahan gaya hidup, misalnya dengan mengubah menu makanan pokok dari beras padi menjadi beras analog yang terbuat dari bahan baku modified cassava flour (MOCAF) dan jagung. Beras analog ini mengandung resistant startch dan serat yang tinggi sehingga indeks glikemiknya rendah, serta mengandung antioksidan senyawa fenolik dan β karoten. Penelitian ini bertujuan mengetahui efek terapi kombinasi beras analog dan glimepirid terhadap kadar MDA pankreas pada tikus DMT 2. Tikus Wistar sebanyak 24 ekor dibagi menjadi kelompok kontrol dan 3 kelompok perlakuan. Pada kelompok perlakuan diberikan diet tinggi lemak selama 17 hari dan diinduksi STZ pada hari ke-15. Pada hari ke-18 kelompok perlakuan dibagi menjadi 3 kelompok masing-masing diberikan pakan standar (KN), diberikan pakan standar dan glimepirid dengan dosis 0,1 mg/KgBB/hari (P1), diberikan pakan beras analog dan glimepirid 0,1 mg/KgBB/hari (P2). Perlakuan tersebut dilakukan selama 21 hari. Pengukuran kadar MDA pankreas menggunakan metode ELISA. Hasil penelitian adalah kadar MDA kelompok kontrol = 0,437; KN = 0,649; P1 = 0,384; P2 = 0,300. Terdapat perbedaan yang signifikan antara KN dan P2. Kesimpulannya adalah kombinasi glimepirid dan beras analog mampu menurunkan kadar MDA pankreas pada tikus DM Tipe 2. Kata Kunci: Diabetes Melitus Tipe 2, MDA Pankreas, Glimepirid, Beras Analog, MOCAF  The prevalence and incidence of type 2 diabetes mellitus (T2DM) in the world continues to increase. This disease is characterized by hyperglycemia, which triggers oxidative stress conditions. One oral therapy that is often used is glimepiride. Controlling hyperglycemia in T2DM sufferers requires lifestyle changes, for example, changing the staple food from rice to analog rice made from modified cassava flour (MOCAF) and corn. This analog rice contains resistant starch and high fiber, so the glycemic index is low. It has antioxidant phenolic compounds and β-carotene. This study aims to determine the effect of rice analog and glimepiride combination therapy on pancreatic MDA levels in T2DM mice. Twenty-four Wistar rats were divided into the control group and three treatment groups. The treatment group was given a high-fat diet for 17 days and induced by STZ on the 15th day. On the 18th day, the treatment group was divided into three groups, each given standard feed (KN), standard feed and glimepiride 0,1 mg/KgBW/day (P1), and analog rice feed and glimepiride 0,1 mg/KgBW/day (P2). This treatment was carried out for 21 days. Measurement of pancreatic MDA levels using the ELISA method. The results in this study were MDA level of control group=0.437; KN=0.649; P1=0.384; P2=0.300. There is a significant difference between KN and P2. Conclusion: The combination of glimepiride and rice analogs can reduce pancreatic MDA levels in T2DM rats.Referensi
Apriliany, F., Cholisah, E., & Erlianti, K. (2022). Efek Pemberian Metformin dan Metformin + Glimepiride Terhadap Kadar HbA1c pada Pasien Diabetes Mellitus Tipe 2. Jurnal Manajemen dan Pelayanan Farmasi, 12(2), 97–106. https://doi.org/10.22146/jmpf.72192
Budiarso, F. S., Suryanto, E., & Yudishtira, A. (2017). Ekstraksi dan Aktivitas Antioksidan dari Biji Jagung Manado Kuning (Zea Mays L.). Pharmacon, 6(3), 302–309. https://doi.org/10.35799/pha.6.2017.16944
Darenskaya, M.A., Kolesnikova, L.I., Kolesnikov, S.I. (2021) Oxidative Stress: Pathogenetic Role in Diabetes Mellitus and Its Complications and Therapeutic Approaches to Correction. Bulletin of Experimental Biology and Medicine, 171(2), 179-189. https://doi.org/10.1007/s10517-021-05191-7.
Hairrudin, Soetjipto, & Handajani, R. (2019). Hypoglycemic Effect of High-Resistant Starch Analog Rice Through Glp-1 and Insulin or High-Resistant Starch Analog Rice Attenuates Blood Glucose Level Through Enhancement of Glp-1 and Insulin. Asian Journal of Pharmaceutical and Clinical Research, 12(8), 172–175. https://doi.org/10.22159/ajpcr.2019.v12i18.33944
IDF, I. D. F. (2021). IDF Diabetes Atlas, 10th Edition. In Journal of Experimental Biology. https://doi.org/10.1242/jeb.64.3.665
Kurt, S., Seker, U., Yazlik M.O., Er, E., Hürkul M.M, Soker, S. (2023). Identification of major phenolic compounds of Aloe vera and its protective effect on ovaries under oxidative stress in diabetic rats. Journal of Research in Pharmacy, 27(2), 652-664. http://dx.doi.org/10.29228/jrp.348
Lv, W., Wang, X., Xu, Q., & Lu, W. (2019). Mechanisms and Characteristics of Sulfonylureas and Glinides. Current Topics in Medicinal Chemistry, 20(1), 37–56. https://doi.org/10.2174/1568026620666191224141617
Nahdi, A. M. T. A., John, A., & Raza, H. (2017). Elucidation of Molecular Mechanisms of Streptozotocin-Induced Oxidative Stress, Apoptosis, and Mitochondrial Dysfunction in Rin-5F Pancreatic β -Cells. Oxidative Medicine and Cellular Longevity, 2017. https://doi.org/10.1155/2017/7054272
Noviasari, S., Kusnandar, F., Setiyono, A., & Budijanto, S. (2015). Beras analog Sebagai Pangan Fungsional dengan Indeks Glikemik Rendah. Jurnal Gizi Pangan, 10(3), 225–232.
Nugraheni, M., Purwanti, S., & Ekawatiningsih, P. (2021). Impact of analog rice derived from different composite flours from tubers, germinated legumes, and cereals on improving serum markers in alloxan-induced diabetic rats. Preventive Nutrition and Food Science, 26(3), 296–306. https://doi.org/10.3746/PNF.2021.26.3.296
Peptide, A. G., & Drucker, D. J. (2018). Review Mechanisms of Action and Therapeutic. Cell Metabolism, 27(4), 740–756. https://doi.org/10.1016/j.cmet.2018.03.001
PERKENI. (2021). Pedoman Pengelolaan dan Pencegahan Diabetes Melitus Tipe 2 Dewasa di Indonesia-2021 PERKENI i Penerbit PB. PERKENI.
Pratiwi, R.Y., Elya, B., Setiawan, H., Solawati, A. (2021). Alterations in Body Weight, Blood Glucose Levels, and Lipid Profiles in High-Fat Diet-Low Dose Streptozotocin-Induced Diabetic Rats. Pharmacognosy Journal, 13(6s), 1562-1567. https://doi.org/10.5530/pj.2021.13.199
Rajlic, S., Treede, H., Münzel, T., Daiber, A., & Duerr, G. D. (2023). Early Detection Is the Best Prevention—Characterization of Oxidative Stress in Diabetes Mellitus and Its Consequences on the Cardiovascular System. Cells, 12(4). https://doi.org/10.3390/cells12040583
Rani, K., Gavel, P., & Bharti, S. (2019). MDA, Oxidative Stress Marker-Role in Diabetic Nephropathy With Special Reference to Type II Diabetes Mellitus Medical Science Keywords MDA, Oxidative Stress Marker-Role in Diabetic Nephropathy With Special Reference to Type II Diabetes Mellitus. Indian Journal of Applied Research, 6(5), 128–130.
Retaningsih, V., & Kora, F. T. (2022). Peningkatan kualitas hidup pasien dm dengan menjaga kadar gula darah. Jurnal Informasi Kesehatan & Administrasi Rumah Sakit (IKARS), 1(2), 50–52. https://doi.org/10.55426/ikars.v1i2.214
Sartika, L., & Rahardiantini, I. (2022). Pengaruh Glimepirid terhadap Penurunan Glukosa Darah pada Mencit Diabetes-Disfungsi Ginjal. Jurnal Ilmiah Medicamento, 8(2), 104–109. https://doi.org/10.36733/medicamento.v8i2.3517
Shawki, H. A., Elzehery, R., Shahin, M., Abo-hashem, E. M., & Youssef, M. M. (2021). Evaluation of some oxidative markers in diabetes and diabetic retinopathy. Diabetology International, 12(1), 108–117. https://doi.org/10.1007/s13340-020-00450-w
Sola, D., Rossi, L., Schianca, G. P. C., Maffioli, P., Bigliocca, M., Mella, R., Corlianò, F., Paolo Fra, G., Bartoli, E., & Derosa, G. (2015). Sulfonylureas and their use in clinical practice. Archives of Medical Science, 11(4), 840–848. https://doi.org/10.5114/aoms.2015.53304
Wronka, M., Krzemińska, J., Młynarska, E., Rysz, J., & Franczyk, B. (2022). The Influence of Lifestyle and Treatment on Oxidative Stress and Inflammation in Diabetes. International Journal of Molecular Sciences, 23(24). https://doi.org/10.3390/ijms232415743
Yaribeygi, H., Noroozadeh, A., Mohammadi, M. T., Johnston, T. P., & Sahebkar, A. (2019). Crocin improves oxidative stress by potentiating intrinsic anti-oxidant defense systems in pancreatic cells during uncontrolled hyperglycemia. Journal of Pharmacopuncture, 22(2), 83–89. https://doi.org/10.3831/KPI.2019.22.010
Zhao, X., Huang, P., & Yuan, J. (2022). Influence of glimepiride plus sitagliptin on treatment outcome, blood glucose, and oxidative stress in diabetic patients. American Journal of Translational Research, 14(10), 7459–7466. http://www.ncbi.nlm.nih.gov/pubmed/36398218%0Ahttp://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=PMC9641479
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