Perbandingan Aktivitas Analgetik Infusa dan Ekstrak Etanol Umbi Akar Tawas Ut (Ampelocissus rubiginosa Lauterb.)
DOI:
https://doi.org/10.20527/jps.v6i2.7349Abstract
ABSTRAK Umbi akar tawas ut (Ampelocissus rubiginosa Lauterb.) secara empiris digunakan untuk mengurangi nyeri. Masyarakat menggunakannya dengan cara meminum air seduhannya. Penelitian ini bertujuan untuk mengetahui perbandingan aktivitas analgetik dari infusa dan ekstrak etanol umbi akar A. Rubiginosa. Uji analgetik dilakukan menggunakan metode geliat (Writhing test) dengan pembanding parasetamol. Tiga puluh ekor mencit dibagi 6 kelompok masing-masing 5 ekor per kelompok. Kelompok I kontrol positif (parasetamol 65,25 mg/kgBB), kelompok II kontrol negatif (Na-CMC), kelompok III infusa A. rubiginosa 25 ml/kgBB, dan kelompok IV ekstrak etanol A. rubuginosa 500 mg/kgBB. Sesudah diberi perlakuan secara per oral sesuai kelompoknya, 30 menit kemudian diinduksi dengan asam asetat secara intraperitoneal. Jumlah geliat dihitung setiap 5 menit setelah pemberian larutan asam asetat 1% dengan selama 1 jam. Hasil penelitian menunjukkan persen proteksi pemberian parasetamol 65,25 mg/kgBB, infusa A. rubiginosa 65,25 mg/kgBB dan ekstrak etanol A. rubiginosa 500 mg/kgBB secara berurutan adalah 76,04; 87,41 dan 63,77%. Dari penelitian dapat disimpulkan bahwa infusa umbi akar A. rubiginosa memiliki aktivitas analgetik yang kuat. Kata kunci: Ampelocissus rubiginosa Lauterb., analgetik, infusa, ekstrak etanol  ABSTRACT Tuberous root of tawas ut (Ampelocissus rubiginosa Lauterb.) empirically used to reduce pain. People use it by drinking boiled water of A. rubiginosa coarse powder. This study aims to determine the comparison of analgesic activity of infusion and ethanol extract of A. rubiginosa tuberous root. Analgesic test was performed using a stretching method (Writhing test) with paracetamol as comparison. Thirty mice were divided into 6 groups of 5 individuals per group. Group I was positive control (paracetamol 65.25 mg / kgBW), negative control group II (Na-CMC), group III A. rubiginosa infusion 25 ml / kgBW, and group IV ethanol extract A. rubiginosa 500 mg / kgBW. After being treated orally according to the group, 30 minutes later induced with acetate acid intraperitoneally. The amount of stretching was calculated every 5 minutes after giving 1% acetic acid solution for 1 hour. The results showed percent protection of paracetamol 65.25 mg / kgBB, A. rubiginosa infusion 65.25 mg / kgBB and ethanol extract A. rubiginosa 500 mg / kgBB was 76.04; 87.41 and 63.77% respectively. From the research it can be concluded that A. rubiginosa root tuber infusion has a strong analgesic activity. Keyword: Ampelocissus rubiginosa Lauterb., analgetic, infusa, ethanol extractReferences
Ali, A.H.A., Rinidar, Armansyah, T., Rosmaidar, Harris, A., dan Dasrul. 2016. Potensi Ekstrak Daun Sernai (Wedelia biflora) Sebagai Antinyeri Pada Mencit (Mus musculus). Jurnal MedikaVeterinaria. 10 : 137-140.
Anwar, K., Widodo, D.F., Nurlely, N., Triyasmono, L., Sudarsono, S. and Nugroho, A.E., Wound Healing Activity of Ethanolic Extract Gel of Tawas Ut Tuber (Ampelocissus rubiginosa L.) in Incisional Model Wistar Rats. Majalah Obat Tradisional, 23(1), pp.30-39.
Astuti, K.I., Anwar, K. dan Biworo, A., 2016. Uji Aktivitas Infusa Akar Tawas Ut (Ampelocissus rubiginosa L.) sebagai Hepatoprotektor Terhadap Mencit Putih Jantan Balb/C yang Diinduksi Karbon Tetraklorida (CCl4). Jurnal Pharmascience, 3(2).
Depkes RI. 1979. Farmakope Indonesia Edisi III. Departemen Kesehatan Republik Indonesia. Jakarta.
Diphalma, J. R. and Digregorio, G. J, 1986. Basic Pharmacology in Medicine. 3th ed. McGraw-Hill Publishing Company : 319-20. New York.
Dipiro, J.T., B.G. Wells, Schwinghammer, T. L., and Dipiro C. V. 2008. Pharmacotherapy: A Pathophysiologic Approach, Seventh Edition. Mc-Graw Hill.
Galani, V.J., and Patel, B.G. 2011. Analgesic and Anti-inflammatory Activity of Argyreia spesiosa and Sphearanthusn indicus in the Experimental Animal. Global Journal of Pharmacology. 5 : 54-59.
Mackay, D., and Miller, A.L. 2003. Nutritional Support for Wound Healing. Alternative Medicine Review. 8: 369-370.
Meliala, L. and Pinzon, R. 2007. Breakthrough in Management of Acute Pain. Dexa Media. 20 : 151-155.
Ningsih, S. dan Nova, R.W. 2014. Kemampuan Efek Sedasi Infusa Umbi Rumput Teki (Cyperus rotundus L) Pada Mencit Jantan Ras Swiss. Indonesian Journal On Medical Science. 1 : 66-73.
Ningsih, D.R., Zusfahair, dan Kartika, D. Identifikasi Senyawa Metabolit Sekunder serta Uji Aktivitas Ekstrak Daun Sirsak Sebagai Antibakteri. Molekul. 11: 101-111.
Puspitasari., Listyawati, S., dan Widiyani, T. 2003. Aktivitas Analgetik Ekstrak Umbi Teki (Cyperus rotundus L.) pada Mencit Putih (Mus musculus L.) Jantan. Biofarmasi. 1: 50-57.
Reynertson, K. A. 2007. Phytochemical Analysis of Bioactive Constituens from Edible Myrtaceae Fruits. Ethnobotany Research & Applications. 3 : 025-035.
Sahu, G. and Gupta, P.K., 2012. A Review On Bauhinia variegata Linn, International Research Journal of Pharmacy. 3 : 48-51.
Sasongko, H., Sugiyarto, S., Efendi, N.R., Pratiwi, D., Setyawan, A.D. and Widiyani, T., 2016. Analgesic Activity of Ethanolic Extracts of Karika Leaves (Carica pubescens) In Vivo. JPSCR: Journal of Pharmaceutical Science and Clinical Research, 1(2), pp.83-89.
Shoibe, M., Chy, M.N.U, Alam, M., Adnan,M., Islam, M.Z., Nihar, S.W., Rahman, N., and Suez, E. 2017. In Vitro and In Vivo Biological Activities of Cissus adnata (Roxb.). Biomedicines. 5 : 1-18.
Suryanto, E. 2013, Potensi Ekstrak Fenolik Buah Pisang Goroho (Musa Spp.) Terhadap Gula Darah Tikus Putih (Rattus norvegicus). Chem. Prog. 6 : 6-10.
Verdam, M.C.D.S., Simplicio, F.G., Andrade, K.C.D., Fernandes, K.L.M., Machado, T.M.F., Silva, M.A.D., Souza, M.P.D., Koolen, H.H.F., Paula, C.D.S., Hirota, B.C.K., Oliveira, V.B.D., Miyazaki, C.M.S., Kalegari, M., Miguel, M.D., Campelo, P.M.S., and Miguel, O.G. 2017. Analgesic, Anti-Inflammatory and Antioxidant Activities of Byrsonima duckeana W. R. Anderson (Malpighiaceae). Hindawi The Scientific World Journal. 2017 : 1-9.
Wang, J. R., Zhou, H., Jiang, Z.H., Wong, Y.F., and Liu, L. 2008. In Vivo Anti-Inflammatory and Analgesic Activities of A Purified Saponin Fraction Derived from The Root of Ilex pubescens. 31 : 643-650.
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