The Antibacterial Activity of Red Dragon Fruit Peel (Hylocereus polyrhizus Britton & Rose) Methanolic Fraction Against Staphylococcus epidermidis and Propionibacterium acnes
DOI:
https://doi.org/10.20527/jps.v8i2.10378Keywords:
Antibacterial, RDFPMF, Staphylococcus epidermidis, Propionibacterium acnesAbstract
Acnes can be caused by Propionibacterium acnes and Staphylococcus epidermidis. Red dragon (Hylocereus polyrhizus B & R) fruit peels is one of natural materials which is assumed to possess an antibacterial activity. The purpose of this study was to determine the antibacterial activity of red dragon fruit peels methanol fraction (RDFPMF) against S. epidermidis and P. acne by disc diffusion Kirby-Bauer method. Red dragon fruit peels were maceration in chloroform and fractionation in methanol. Clindamycin 4µg/disk was used as positive control and DMSO 10% was used as negative control. This research showed that the RDFPMF has antibacterial activity against P. acnes which the average of inhibition zones obtained from the concentrations from 100, 50, and 25 mg/mL respectively were 10.5 ± 0.50 mm, 10.0 ± 0.00 mm, and 8.5 ± 0.50 mm. The RDFPMF has no inhibition zone toward S. epidermidis. The red dragon fruit peels methanol fraction has antibacterial activity on P. acnes by disc diffusion method (Kirby-Bauer test).References
Arum, Y.P., Supartono, and Sudarmin. (2012). Isolasi dan Uji Daya antimikroba ekstrak daun kersen (Muntingia Calabura). Jurnal MIPA UNNES, 35(2).
Azeredo, H.M.C. (2009). Betalain: properties, sources, applicaions, and stabily – a review. Int. Journal Food Science Technol, 44(12), 2365-2376.
Cowan, M. (1999). Plant product as antimicrobial agent. Clinical Microbiology Reviews, 12(4), 564-582.
Geidam, Y.A., H. Usman, M.B., Abubakar, & B. Ibrahim. (2007). Effects of aqueous leaf extracts of Psidium guajava on bacteria isolated from the navel of day-old chicks. Research Journal of Microbiology, 2(12), 960-965.
Hafez, K.A., Mahran, A.M., Hofny, E.R.M., Mohammed, K.A, Darweesh, A.M., Aal, A. (2009). The impact of acne vulgaris on the quality of life and psychologic status in patients from upper egypt. Int. Journal Dermatology, 48(3), 280-5.
Hopp, K.H., Cunningham, L.V., Bromel, M.C., Schermeister, L.J. Wahba,& Khalil S.K. (1976). In vitro antitrypanosomal activity of certain alkaloids against trypanosoma lewisi. Lloydia, 39, 375–377.
Jamilah, B., Shu, C.E., Kharidah, M., Dzulkify, M.A., & Noranizan, A. (2011). Physicochemical characteristics of red pitaya (Hylocereus polyrhizus) peel. Int. Food Research Journal, 18, 279-286.
Katzung, B.G. (2004). Basic and clinical pharmacology (9th ed.). USA: Mc Graw Hill.
Khalili, M.A., Abdullah, A.B., Abdul, M.A. (2012). Antibacterial activity of flesh and peel methanol fractions of red pitaya, white pitaya and papaya on selected food microorganism. Int. Journal of Pharmacy and Pharmaceutical Science, 4(3), 185-190.
Kim, H., Choi, H.K., Moon, J.Y., Kim, Y.S., Mosaddik, A., Cho, S.K. (2011). Comparative antioxidant and antiproliferative activities of red and white pitayas and their correlation with flavonoid and polyphenol content. J of Food Science, 76(1).
Kumar, G.S., Jayaveera, K.N., Ashok, K.C.K., Sanjay, U.P., Swamy, B.M.V., Kishore, K.V.K.. (2007). Antimicrobial effect of indian medicinal plants against acne-inducting bacteria. Tropical Journal Pharmaceutical Research, 6(2), 717-723.
Leyden, J.J. (2001). Current issue in antimicrobial therapy for the treatment of an acnes. Journal Eur Dermatol Venerol, 15(3), 51-55.
Livermore, D.M., Struelens, M., Amorim, J., et al. (2002). Multicentre evaluation of the VITEK 2 advanced expert System for interpretive reading of antimicrobial resistance test. Journal Antimicrob Chem other, 49, 289-300.
Luo, H., Cai, Y., Peng, Z., Liu, T., Yang, S. (2014). Chemical composition and in vitro evaluation of the cytotoxic and antioxidant activities of supercritical carbon dioxide extracts of Pitaya (dragon fruit) Peel. Chemistry Central Journal, 8(1), 2-7.
Marbun, E.D., Sapitri, A., dan Asfianti, V. (2021). Uji aktivitas ekstrak etanol, fraksi etil asetat, fraksi n-heksan daun sofo-sofo (Acmella cf) terhadap bakteri propionibacterium acnes dan staphylococcus epidermis sebagai Antibakteri.
Napoleon, A., Swetha, S., dan Angajala, G. (2013). In-vitro anti oxidant and anti bacterial studies of betacyanin isolated from bougenvilla glabra. Int J Pharm Pharm Sci, 59(4), 84-87.
Nurliyana, R, Syed, Z.I., Mustapha, S.K., Aisyah, M.R., Kamarul, R.K. (2010). Antioxidant study of pulps and peels of dragon fruits: a comparative study. Int. Food Research Journal Malaysia, p., 367-375.
Oprica, C. (2004). Antibiotic resistant propionibacterium acnes on the skin of patient with moderate to severe acne. J of Pharmacology, 10(3), 155-164.
Pelczar, M.J., & Chan, E.C.S. (1986). Dasar-dasar mikrobiologi 2. Diterjemahkan oleh Hadioetomo RS, Imas T, Tjitrosomo SS, Angka SL. Jakarta: Penerbit Universitas Indonesia.
Phillipson, J.D., & O’Neill, M.J. (1987). New leads to the treatment of protozoal infections based on natural product molecules. Acta Pharmaceutical Nord, 1, 131-44.
Pratiwi, S.T. (2008). Mikrobiologi farmasi. Yogyakarta: Penerbit Erlangga.
Ridwan, A.R.S. (2012). Stabilitas ekstrak kulit buah naga merah (Hylocereus polyrhizus) sebagai antimikroba terhadap mikroba patogen pangan. The Johanes Oentoro Library UPH.
Swanson, I.K. (2003). Antibiotic resistance of propionibacterium acnes in acne vulgaris. Dermatol Nurs, 15(4), 5359-361.
Tahany, M.A., Hegazy, A.K., Sayed, A.M., Kabiel, H.F., El-Alfy, & El-Komy, S.M. (2010). Study on combined antimicrobial activity some biologically active constituents from wild Moringa peregrina Forssk. Journal of Yeast and Fungal Research, 1(1), 015-024.
Valsaraj, R., Pushpangadan, P., Smith, U.V., et al. (1997). Antimcrobial screening of selected medisinal plants from India. Journal Ethnopharmavol, 58, 75-83.
Wu, L.C., Hsu, H.W., Chen, Y.C., Chiu, C.C., Lin, Y.I., Annie, H.J. (2006). Antioxidant and antiproliferative activities of red pitaya. Food Chemistr, 95(2).
Yang, H., J.G. Boersma, M. You, B.J. Buirchell, & M.W. (2004). Sweetingham. Development and implementation of a sequence-specific PCR marker linked to a gene conferring resistance to anthracnose disease in narrow-leafed lupin (Lupinus angustifolius L.). Molecular Breeding, 14, 145-151.
Zainoldin, K.D. (2012). The effect of Hylocereus polyrhizus and Hylocereus undatus on physicochemical, proteolysis and antioxidant activity in yogurt. Int. Journal of Biological and Life Science, 8(2), 93-98.
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